Tuesday, February 26, 2013

Cell and Haemocytometer

Experiment: Yeast Cells and Haemocytometer
Research Question: What is the cast of barm cells per cm³ in different dilutions mover of yeast intermission?
Hypothesis: The high the dilutions factor of yeast reprieve, the lesser the takings of yeast cells. When the dilution factor increases means that the volume of the distilled water is higher than the number of yeast cells suspended in the solution. So the number of yeast cells will decreases.
Variables:
| | Units| Range|
Independent variable| Dilution factor of yeast suspension | | 2-4|
Dependent variable| The number of yeast cells in one primary square| | |

Controlled variables| Units| Method for reserve/possible effects on results|
(1) good deal of distilled water| cm³| 9ml of distilled water is used to diluted the yeast suspension|
(2) Volume of yeast suspension| cm³| 1ml of yeast suspension is taken from each of the test tubes during the dilution series|
(3) Magnification of objective genus Lens| | 10× of objective lens is used to focus on the primary square. Since different power of lenses have different region of view.

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(4) Temperature| ? C| The experiment is carried out in the laboratory of inhabit temperature of 28 ? C.|
(5) Pressure| atm| The experiment is carried out in the laboratory under 1 atmospheric pressure.|

Materials and Apparatus: identify to handbook from page 163-167
Methodology:
1. The haemocytometer is cleaned with alcohol and wiped with tissues.
2. The slide is moistened victimization damp tissues.
3. The special coverslip is then pushed on to the slide and touch down on the edges of the coverslip at the same time.
4. Then the yeast suspension is shaken gently.
5. A dropper is inserted into the yeast suspension and the liquid is rose into it.
6. The dropper is...If you want to get a full essay, order it on our website: Orderessay



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